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mouse heart lysate  (Novus Biologicals)


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    Novus Biologicals mouse heart lysate
    Mouse Heart Lysate, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+heart+lysate/pm35860280-60-33-36?v=Novus+Biologicals
    Average 92 stars, based on 5 article reviews
    mouse heart lysate - by Bioz Stars, 2026-07
    92/100 stars

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    FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat <t>tissue</t> extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell <t>lysate</t> (TLR10) and <t>mouse</t> <t>heart</t> whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.
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    FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.

    Journal: Biology of reproduction

    Article Title: Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.

    doi: 10.1095/biolreprod.106.059410

    Figure Lengend Snippet: FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.

    Article Snippet: Mouse heart tissue lysate (40102) and Ramos cell lysate (40175) was from Imgenex.

    Techniques: Western Blot, Control, Positive Control, Transformation Assay